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The Theory of Contrast
Any microscopic picture includes both background illumination and a specimen image.
Contrast is the juxtaposition of form and color to demonstrate a distinction between the image of the specimen and the backdrop lighting.
To improve visibility, we alter the contrast in the microscopic image.
When there is no bright or dark dividing barrier around the specimen’s image, the threshold value of visibility when the human eye can only discern a difference between the item and the backdrop is 2%.
The 2% contrast rating is based on first-rate optical components, microscope alignment, and visual acuity.
As the resolution limit approaches, the necessity for proper contrast becomes more critical.
This drop, in contrast, usually occurs while investigating the intricacy of minute features within the specimen’s body.
We are frequently faced with the dilemma of maximizing both the visibility and detail of specimens deficient in contrast, particularly in biological microscopy.
This demonstrates the significance of selectivity: to identify detail, we require at least some regional variations in contrast within the object.
How Contrast is Controlled in LYNX Phase Contrast Microscopes
Proper control of three fundamental parameters governs its use in any compound microscope.
- Resolving power
- Contrast
- Magnification
Because of the limitations imposed by the microscope’s construction on resolving power and magnification, only the second essential parameter, contrast, is under the microscopist’s direct control.
An Observer may change the contrast to investigate many different sorts of specimens, or a single sample can be studied using several contrast enhancement methods to extract the most information about it.
Contrast can be increased by inserting appropriate apertures or filters into the optical path, either in the lighting system (dark ground or Rheinberg illumination) or in conjugate planes in the imaging system (e.g., for phase contrast, differential interference contrast, or polarized light microscopy).
The condenser is a small, adjustable piece of equipment for changing the light and adding the required apertures or filters for picture contrast enhancement. A condenser is the only significant optical component that can be entirely modified by the user, and it influences both the kind and quality of lighting, as well as the image’s visibility.
The LYNX phase contrast inverted microscope is intended to exploit phase variations between items in a specimen and their surroundings. However, diffraction from the specimen and a phase difference is required for the phase contrast microscope to function.
LYNX Inverted Microscope offers super long working distance plan achromatic objectives 4X/10X/40X together with 20X phase contrast objective for a standard recommendation, provide clear and high contrast image.
We also offer a phase contrast kit consisting of a dedicated phase contrast plate, working with phase contrast objectives, ensuring the best contrast image of a living cell or other undyed samples.
LYNX Phase Contrast Microscope comes with various accessories, including cameras, imaging software for realistic and professional photographs, and a specially built kit to preserve time records.
To visualize on a larger screen, simply connect your smartphone via USB to the Image projection system. Our several high-quality camera attachments for digital microscope systems produce digital images with natural color technology and robust image analysis tools.
Discover life beyond the naked eye with a wide range of LYNX microscopes ranging from training and routine tasks to high-end system solutions in life science and research applications.
We offer unparalleled image resolution, high-quality cameras, optics, and associated accessories commonly used in life science and industrial solutions. For more information contact us at 022 2207 1828.[/vc_column_text][/vc_column][/vc_row]